Chromatography Quality and Multiple signal detection

Chromatography Quality and Multiple signal detection

Mass Spectrometry (LC-MS & Maldi), Ultraviolet, Radio and Fluorescent 

WebQuant can handle signals from different detector types, performing peak finding in addition to the Mass Spectra chromatogram. The user can select Ultraviolet, Radio chromatography and/or Fluorescence from the analog channel or from external data file sources. All these signals are then analyzed following different peak quality assessment that can also be used to filter chromatographic peaks and automatized using the WebQuant Macro system: 

Peak quality analysis
  • Difference between observed and calculated m/z (amu, ppm): For the MS signal the system computes the difference between the observed and the computed m/z. The Observed m/Z considers the m/z finds at the different scans and derived a value which has been compared to the vendor software package to consider effects like peak saturation and loss of accuracy at the top of the peak.  
  • Comparison to the blank: The system is calculating the ratio between the area of the peak found in the incubation sample under analysis and the black in the case of the MS signal. For Ultraviolet and Fluorescent signal this comparison is done during the peak finding together with a base line correction.