LipostarMSI is a software for mass spectrometry imaging (MSI) lipidomics that supports both untargeted and targeted data analysis, distributed as desktop application. 

The workflow supported by LipostarMSI covers all the steps required for MSI data analysis: raw data processing, histological coregistration, manual drawing or importing of regions annotated by pathologists, image visualization, a variety of univariate and multivariate image and spectral analysis tools, co-localization analysis, and automated lipids, metabolites and drug metabolites annotation, including MS/MS-based identification. 



Data import

  • Vendor, Instrument & Acquisition neutral analysis  
  • Annotated ROI import 
  • Import of MS/MS spectra from LC-MS acquisitions 


  • Peak detection and pre-processing 
  • Annotation and identification 
  • Biochemical Pathways


  • Targeted and untargeted omics analysis  
  • Lipids, metabolites and drug metabolites  

Image analysis

  • Denoise, contrast enhancement, normalization
  • Single and overlayed ion density images visualization
  • Coregistration with H&E

Data analysis

  • Segmentation 
  • Co-localization 
  • Principal Component Analysis 
  • Univariate and multivariate ROI analysis 


  • Background processing of computational intensive tasks
  • Optimized and parallelized algorithms
  • Automatic report generation

System requirements & Installation

  • System requirements
  • Installation instructions

Pharma –  Data Analysis –  Imaging



Life Sciences –  Imaging Metabolites

  • Oniro: for HTS rapifleX data analysis 
  • Pyxis suite: for multi-purpose MSI, including ion mobility MSI 
  • Lipostar: for LC-MS lipidomics  
  • MARS: for LC-MS metabolomics  
  • Action: for affinity measurement  

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