Pyxis

Pyxis is a software for ion mobility mass spectrometry imaging (IMS-MSI), that supports both untargeted and targeted data analysis, distributed as desktop application.  

The workflow supported by Pyxis covers all the steps required for IMS-MSI data analysis: raw data processing, histological coregistration, image and mobilogram visualization, manual drawing or importing of regions annotated by pathologists, image visualization, exploratory data analysis (segmentation, PCA, colocalization), automatic report generation, a variety of univariate and multivariate image and spectral analysis tools, and automated metabolites annotation, including CCS- and MS/MS-based identification. A number of functions to export results in open format are also available. 

Data import

  • Vendor, Instrument & Acquisition neutral analysis  
  • Annotated ROI import 

Information

  • Peak detection and pre-processing in 3D (m/z, CCS, intensity) 
  • Annotation and identification 
  • Denoise, contrast enhancement, normalization 

Data analysis

  • Targeted and untargeted analysis  
  • Segmentation 
  • Co-localization 
  • Principal Component Analysis 
  • Univariate and multivariate ROI analysis 

Omics

  • Targeted and untargeted analysis  
  • Metabolites  

Image visualization

  • Denoise, contrast enhancement, normalization 
  • Single and overlayed images visualization 
  • Coregistration with H&E, fluorescence and other imaging modalities 

Automation

  • Background processing of computational intensive tasks 
  • Optimized and parallelized algorithms  
  • Automatic report generation 

System requirements and installation

  • System requirements 
  • Installation instructions 

Pyxis Training documents

Videos:
  • Pyxis MSI: for multi-purpose MSI data analysis 
  • LipostarMSI: for MSI lipidomics data analysis 
  • Oniro: for HTS rapifleX data analysis 
  • Lipostar: for LC-MS lipidomics  
  • MARS: for LC-MS metabolomics  
  • Action: for affinity measurement 

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