Pyxis is a software for ion mobility mass spectrometry imaging (IMS-MSI), that supports both untargeted and targeted data analysis, distributed as desktop application.
The workflow supported by Pyxis covers all the steps required for IMS-MSI data analysis: raw data processing, histological coregistration, image and mobilogram visualization, statistical analysis and automated metabolites identification.
Key features
Data import
- Bruker timsTOF flex
Information
- Peak detection and pre-processing, both on the m/z and mobility domains
- Spectral and image analysis
- Statistical Analysis: Segmentation, PCA, Colocalization
- Annotation and identification
Image Analysis
- Denoise, contrast enhancement, normalization
- Single and overlayed ion density images visualization
- Coregistration
Read more:
- Coregistration and transparency customization of multiple optical images for each dataset
- Possibility to cluster and visualize isobars images but with different mobility values
Automation
- Background processing of computational intensive tasks
- Optimized and parallelized algorithms
System requirements & Installation
- System requirements
- Installation instructions
Videos:
- Ion Mobility MSI: Adding A New Dimension To Spatial Metabolomics
- Practical Applications for Drug Discovery 2020 Webinar. Sara Tortorella, Jens Soltwisch
Posters:
- Adding a new dimension to spatial omics: Pyxis, Comprehensive software for ion mobility MS imaging data analysis
- IMSC 2022. Sara Tortorella, Giuseppe Arturi, Giulia Sorbi, Sebastian Bessler, Gabriele Cruciani
